Unlimited [Science Book] ☆ Over-Expression of Human Sirt1 Prevents Ageing-Induced Endothelial Dysfunction: Enos Dependent and Independent Mechanisms - by Jie Li 李杰 ✓

By Jie Li 李杰 | Comments: ( 524 ) | Date: ( Apr 05, 2020 )

This dissertation, Over expression of Human SIRT1 Prevents Ageing induced Endothelial Dysfunction ENOS Dependent and Independent Mechanisms by Jie, Li, , was obtained from The University of Hong Kong Pokfulam, Hong Kong and is being sold pursuant to Creative Commons Attribution 3.0 Hong Kong License The content of this dissertation has not been altered in any way.This dissertation, Over expression of Human SIRT1 Prevents Ageing induced Endothelial Dysfunction ENOS Dependent and Independent Mechanisms by Jie, Li, , was obtained from The University of Hong Kong Pokfulam, Hong Kong and is being sold pursuant to Creative Commons Attribution 3.0 Hong Kong License The content of this dissertation has not been altered in any way We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation All rights not granted by the above license are retained by the author Abstract In blood vessel of mature animal, endothelial cells remain quiescent for years, before apoptosis and being replaced by newly generated endothelial cells During aging, this turnover process is accelerated and the fast generated endothelial cells become dysfunctional Endothelial dysfunction in blood vessel is characterized by the imbalanced production of endothelium dependent relaxing factors EDRF and endothelium dependent contracting factors EDCF The NAD dependent deacetylase SIRT1 is an anti aging protein with therapeutic potential for aging related cardiovascular diseases Endothelium specific over expression of human SIRT1 promotes endothelium dependent vasodilatation and endothelium selective inhibition of human SIRT1 inhibit it It is accepted that SIRT1 plays a protective role in endothelium dysfunction However, the underlying mechanisms remain unclear In the present study, the endothelial functions of a transgenic mouse model with endothelium selective over expression of human SIRT1 hSIRT1 were evaluated and compared with those of wild type mice Aging induced deterioration in endothelium dependent vasodilatation was observed in wild type but not hSIRT1 mice Endothelium specific over expression of SIRT1 prevented aging induced reduction of NO bioavailability in aortae, without changing endothelial nitric oxide synthase eNOS expression levels Enhanced phosphorylation of eNOS at serine 1177 was detected in hSIRT1 mice aorta In the presence of N Nitro L arginine methyl ester L NAME , the nitric oxide synthase NOS inhibitor, EDCF induced contraction to acetylcholine was significantly decreased in carotid arteries of hSIRT1 mice Cyclooxygenase 2 COX 2 expression was induced by aging in wild type mice but not in hSIRT1 mice Thus, both the augmented NO bioavailability and the reduced production of COX 2 derived EDCF in hSIRT1 mice enhanced their endothelial function To further explore the eNOS independent mechanism underlying the vasoprotective role of SIRT1, the eNOS deficient and hSIRT1 endothelium specific over expression eNOS hSIRT1 mice was generated Decreased endothelium dependent contraction to acetylcholine was observed in both the carotid artery and aorta of eNOS hSIRT1 mice, when compared to the controlled eNOS deficient mice Besides, ATP induced endothelium dependent contraction, which was COX dependent, was also decreased in aortae of eNOS hSIRT1 mice Thus, the improved endothelial function induced by the endothelium selective overexpression of SIRT1 was partly attributed to the reduced COX function, independent of eNOS signaling pathway.In summary, endothelium selective overexpression of human SIRT1 prevented aging induced impairment of endothelial function via both eNOS dependent and independent mechanisms DOI 10.5353 th_b5194769 Subjects Vascular endothelium AgingSirtuins


  • Title: Over-Expression of Human Sirt1 Prevents Ageing-Induced Endothelial Dysfunction: Enos Dependent and Independent Mechanisms
  • Author: Jie Li 李杰
  • ISBN: 9781361340714
  • Page: 264
  • Format: Hardcover

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Jie Li 李杰

Jie Li 李杰 Is a well-known author, some of his books are a fascination for readers like in the Over-Expression of Human Sirt1 Prevents Ageing-Induced Endothelial Dysfunction: Enos Dependent and Independent Mechanisms book, this is one of the most wanted Jie Li 李杰 author readers around the world.



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  • Unlimited [Science Book] ☆ Over-Expression of Human Sirt1 Prevents Ageing-Induced Endothelial Dysfunction: Enos Dependent and Independent Mechanisms - by Jie Li 李杰 ✓
    264 Jie Li 李杰
  • thumbnail Title: Unlimited [Science Book] ☆ Over-Expression of Human Sirt1 Prevents Ageing-Induced Endothelial Dysfunction: Enos Dependent and Independent Mechanisms - by Jie Li 李杰 ✓
    Posted by:Jie Li 李杰
    Published :2020-01-25T03:51:20+00:00